Comparison of unprocessed silk cocoon and silk cocoon middle layer membranes for guided bone regeneration
김성곤, 김민근, 권해용, 조유영, 이광길, 이정근,
소속 상세정보
김성곤 ( Kim Seong-Gon ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
김민근 ( Kim Min-Keun ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
권해용 ( Kweon Hae-Yong ) - National Academy of Agricultural Science Sericultural and Apicultural Materials Division
조유영 ( Jo You-Young ) - National Academy of Agricultural Science Sericultural and Apicultural Materials Division
이광길 ( Lee Kwang-Gill ) - National Academy of Agricultural Science Sericultural and Apicultural Materials Division
이정근 ( Lee Jeong-Keun ) - Ajou University School of Medicine Department of Oral and Maxillofacial Surgery
Abstract
Background: Silk cocoon is composed of multiple layers. The natural silk cocoon containing all layers was cut as a rectangular shape as defined as total group. The inner and outermost layers were removed from the total group and the remained mat was defined as the middle group. The objectives of this study was to compare the total group with the middle group as a barrier membrane for the guided bone regeneration.
Methods: The effects of these materials on the cellular proliferation and alkaline phosphatase (ALP) expression of MG63 cells were explored. For comparing bone regeneration ability, bilateral bone defects were created in calvarial areas in ten adult New Zealand white rabbits. The defects were covered with silk membranes of the middle group, with silk membrane of the total group used as the control on the contralateral side. The defects were allowed to heal for 4 and 8 weeks. Micro-computerized tomography (μCT) and histological examination were performed.
Results: The middle group exhibited a higher MTT value 48 and 72 h after treatment compared to the total group. ALP expression was also higher in the middle group. The results of μCT and histologic examination showed that new bone formation was significantly higher in the middle group compared to the total group 8 weeks postoperatively (P?
Conclusions: In conclusion, the middle layer of the silk cocoon supports guided bone regeneration better than unprocessed silk cocoon.
키워드
Guided bone regeneration; Silk cocoon; Membrane; Bone defect
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